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Dynamic metabolomic prediction from genetic variation
Nemčeková, Petra ; Weckwerth, Wolfram (referee) ; Schwarzerová, Jana (advisor)
Hordeum vulgare, tak ako mnoho ďalších plodín, trpí redukovaním genetickej rôznorodosti spôsobeným klimatickými zmenami. Preto je potrebné zlepšiť účinnosť jeho kríženia. Oblasť záujmu sa v poslednej dobe obracia na výskum nepriamych selekčných metód založených na výpočetných predikčných modeloch. Táto práca sa zaoberá dynamickou metabolomickou predikciou založenou na genomických dátach, ktoré pozostávajú z 33,005 jednonukleotidových polymorfizmov. Metabolomické dáta zahŕňajú 128 metabolitov 25 rodín Halle exotického jačmeňa. Hlavným cieľom tejto práce je vytvoriť metabolomické predikcie dynamických dát pomocou rôznych metód, ktoré boli vybrané na základe rôznych publikácií. Vytvorené modely napomôžu predikcii fenotypu alebo odhaleniu dôležitých vlastností rastliny Hordeum vulgare.
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Dynamic metabolomic prediction from genetic variation
Nemčeková, Petra ; Weckwerth, Wolfram (referee) ; Schwarzerová, Jana (advisor)
Hordeum vulgare, tak ako mnoho ďalších plodín, trpí redukovaním genetickej rôznorodosti spôsobeným klimatickými zmenami. Preto je potrebné zlepšiť účinnosť jeho kríženia. Oblasť záujmu sa v poslednej dobe obracia na výskum nepriamych selekčných metód založených na výpočetných predikčných modeloch. Táto práca sa zaoberá dynamickou metabolomickou predikciou založenou na genomických dátach, ktoré pozostávajú z 33,005 jednonukleotidových polymorfizmov. Metabolomické dáta zahŕňajú 128 metabolitov 25 rodín Halle exotického jačmeňa. Hlavným cieľom tejto práce je vytvoriť metabolomické predikcie dynamických dát pomocou rôznych metód, ktoré boli vybrané na základe rôznych publikácií. Vytvorené modely napomôžu predikcii fenotypu alebo odhaleniu dôležitých vlastností rastliny Hordeum vulgare.
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Mitochondrial DNA variability in the Central European populations.
Veselá, Monika ; Černý, Viktor (advisor) ; Munclinger, Pavel (referee)
The aim of this thesis was to evaluate the genetic diversity of mtDNA of populations in Central Europe and to assess its relation to geographic distances and/or linguistic affiliation of individual populations. For this purpose, HVS-1 mtDNA segments of 194 individuals of 6 Slovak populations were sequenced. Basic parameters of genetic diversity were estimated for obtained sequences, and compared with similar data published by other authors (4 798 sequences of HVS-1 mtDNA segment of 42 population units in Central Europe in total). Intra-population analysis revealed a high level of gene and nucleotide diversity of studied populations, with values clearly decreasing from north to south. While the Slovak populations were bearing the highest rates of genetic diversity, the lowest values were detected in the Croatian populations. The results of ΦST distances point to a certain similarity of the Slavic population (Slovakia, Czech Republic, Poland, Slovenia, Serbia, Bosnia and Herzegovina), Germanic (Germany, Austria, Switzerland) as well as some groups from Hungary and Romania. As the most distant appeared especially the population of Croatia, which differed from almost all populations included in the study. AMOVA showed that the geographic division of population explains the layout of genetic variation...
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Genetic variation in North American crayfish species introduced to Europe and the prevalence of the crayfish plague pathogen in their populations
Filipová, Lenka ; Petrusek, Adam (advisor) ; Diéguez-Uribeondo, Javier (referee) ; Trontejl, Peter (referee)
- ABSTRACT (IN ENGLISH) - Biological invasions by crustaceans represent a serious threat for native species in Europe. In my thesis I focus on non-indigenous freshwater crayfish introduced to Europe and their parasite Aphanomyces astaci, the pathogen of the crayfish plague. The thesis consists of four already published first-author papers (chapters I, II, IV and V), two first-author manuscripts (chapters III and VI), and one paper which I co-authored (chapter VII). The first part (chapters I-V) focuses on genetic variation in North American crayfish introduced to Europe. We showed that in two crayfish species, both successful invaders in Europe, genetic variation differs significantly, reflecting their different colonization histories on the continent. The spiny-cheek crayfish Orconectes limosus was likely introduced to Europe just once, in small numbers (90 individuals). Variation at the mitochondrial DNA (mtDNA) level in the spiny-cheek crayfish in Europe is much lower compared to North America (chapter I), although some variation was revealed by nuclear markers in its Central European populations (chapter II). In contrast, the signal crayfish Pacifastacus leniusculus was introduced to Europe several times, in large numbers. Its European populations are highly diverse genetically and belong to a single...
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Morphometric and genetic analysis of group \kur{Centaurea macroptilon}
OTISKOVÁ, Veronika
Centaurea macroptilon group includes taxa Centaurea macroptilon, Centaurea oxylepis, Centaurea indurata and hybrids. In this master thesis we compared morphological and genetic variation among taxa of this group. Based on the results of morphometric and molecular analyzes we re-considered current taxonomic concept of studied species and suggested changes.
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(A)sexual Life of Liverworts
HOLÁ, Eva
This thesis comprises of two published papers and one accepted manuscript, focused on various aspects of liverwort reproduction. Treated aspects include patterns of asexual reproduction, sex ratio and sex-specic pattern in vegetative growth, and patterns of genetic variation and spatial genetic structure of populations differing in availability of substrate on localities and the population connectivity, and consequently in size, density, and prevailing reproductive mode. These characteristics were studied on representatives of the family Scapaniaceae s.l., belonging to the largest liverwort order Jungermanniales. The results showed that asexual propagules were formed and present in course of the whole growing season and can be considered as a sufficient substitution for sexual reproduction. In contrast with the female-biased sex ratio observed earlier in most dioicous bryophytes, unexpectedly high male-biased sex ratio was observed in the aquatic liverwort, which was speculated to represent a strategy to overcome sperm dilution in aquatic environment. In addition, no size differences between female and male shoots were detected, although the evidence for higher cost of sexual reproduction in females was found. The study of population genetic structure has shown that even small and predominantly asexually reproducing populations are important sources of genetic variation. However, we were able to demonstrate notably low levels of gene flow among populations where habitat fragmentation poses a significant barrier to dispersal of diaspores. The fine scale study of spatial genetic structure revealed a strong aggregation of genotypes, particularly in smaller populations, and at the same time showed that asexual reproduction is an efficient mean of maintaining the populations at not only the short distances, given the spatial extent of clones spanning dozens of meters.
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